AustralieFrV1, Main, Exploration, bibRecord, 000244

The aliphatic amidase AmiE is involved in regulation of Pseudomonas aeruginosa virulence

Identifieur interne : 000244 ( Main/Exploration ); précédent : 000243; suivant : 000245

The aliphatic amidase AmiE is involved in regulation of Pseudomonas aeruginosa virulence

Auteurs : Thomas Clamens [France] ; Thibaut Rosay [France] ; Alexandre Crépin [France] ; Teddy Grandjean [France] ; Takfarinas Kentache [France] ; Julie Hardouin [France] ; Perrine Bortolotti [France] ; Anke Neidig [Allemagne] ; Marlies Mooij [Irlande (pays)] ; Mélanie Hillion [France] ; Julien Vieillard [France] ; Pascal Cosette [France] ; Joerg Overhage [Allemagne] ; Fergal O Ara [Irlande (pays), Australie] ; Emeline Bouffartigues [France] ; Alain Dufour [France] ; Sylvie Chevalier [France] ; Benoit Guery [France] ; Pierre Cornelis [France] ; Marc G. J. Feuilloley [France] ; Olivier Lesouhaitier [France]

Source :

Scientific Reports [ 2045-2322 ] ; 2017.

RBID : PMC:5259723

Abstract

We have previously shown that the eukaryotic C-type natriuretic peptide hormone (CNP) regulates Pseudomonas aeruginosa virulence and biofilm formation after binding on the AmiC sensor, triggering the amiE transcription. Herein, the involvement of the aliphatic amidase AmiE in P. aeruginosa virulence regulation has been investigated. The proteome analysis of an AmiE over-producing strain (AmiE⁺) revealed an expression change for 138 proteins, including some that are involved in motility, synthesis of quorum sensing compounds and virulence regulation. We observed that the AmiE⁺ strain produced less biofilm compared to the wild type, and over-produced rhamnolipids. In the same line, AmiE is involved in P. aeruginosa motilities (swarming and twitching) and production of the quorum sensing molecules N-acyl homoserine lactones and Pseudomonas Quinolone Signal (PQS). We observed that AmiE overproduction reduced levels of HCN and pyocyanin causing a decreased virulence in different hosts (i.e. Dictyostelium discoideum and Caenorhabditis elegans). This phenotype was further confirmed in a mouse model of acute lung infection, in which AmiE overproduction resulted in an almost fully virulence decrease. Taken together, our data suggest that, in addition to its role in bacterial secondary metabolism, AmiE is involved in P. aeruginosa virulence regulation by modulating pilus synthesis and cell-to-cell communication.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5259723

DOI: 10.1038/srep41178
PubMed: 28117457
PubMed Central: 5259723

Affiliations:

Allemagne, Australie, France, Irlande (pays)

Le document en format XML

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 virulence</title>
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, Evreux,<country>France</country>
</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
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<author><name sortKey="Cosette, Pascal" sort="Cosette, Pascal" uniqKey="Cosette P" first="Pascal" last="Cosette">Pascal Cosette</name>
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, Mont-Saint-Aignan,<country>France</country>
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<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
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<author><name sortKey="Overhage, Joerg" sort="Overhage, Joerg" uniqKey="Overhage J" first="Joerg" last="Overhage">Joerg Overhage</name>
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, PO Box 3640, Karlsruhe,<country>Germany</country>
</nlm:aff>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
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<author><name sortKey="O Ara, Fergal" sort="O Ara, Fergal" uniqKey="O Ara F" first="Fergal" last="O Ara">Fergal O Ara</name>
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<country xml:lang="fr">Irlande (pays)</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
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</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
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, Evreux,<country>France</country>
</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Dufour, Alain" sort="Dufour, Alain" uniqKey="Dufour A" first="Alain" last="Dufour">Alain Dufour</name>
<affiliation wicri:level="1"><nlm:aff id="a2"><institution>Univ. Bretagne-Sud, EA 3884, LBCM, IUEM</institution>
, Lorient,<country>France</country>
</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Chevalier, Sylvie" sort="Chevalier, Sylvie" uniqKey="Chevalier S" first="Sylvie" last="Chevalier">Sylvie Chevalier</name>
<affiliation wicri:level="1"><nlm:aff id="a1"><institution>Laboratory of Microbiology Signals and Microenvironment LMSM EA 4312, Normandie Univ, UNIROUEN</institution>
, Evreux,<country>France</country>
</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Guery, Benoit" sort="Guery, Benoit" uniqKey="Guery B" first="Benoit" last="Guery">Benoit Guery</name>
<affiliation wicri:level="1"><nlm:aff id="a3"><institution>Univ. Lille, CHU Lille, EA 7366 - Recherche Translationnelle: relations hôte pathogènes</institution>
, Lille,<country>France</country>
</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Cornelis, Pierre" sort="Cornelis, Pierre" uniqKey="Cornelis P" first="Pierre" last="Cornelis">Pierre Cornelis</name>
<affiliation wicri:level="1"><nlm:aff id="a1"><institution>Laboratory of Microbiology Signals and Microenvironment LMSM EA 4312, Normandie Univ, UNIROUEN</institution>
, Evreux,<country>France</country>
</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Feuilloley, Marc G J" sort="Feuilloley, Marc G J" uniqKey="Feuilloley M" first="Marc G. J." last="Feuilloley">Marc G. J. Feuilloley</name>
<affiliation wicri:level="1"><nlm:aff id="a1"><institution>Laboratory of Microbiology Signals and Microenvironment LMSM EA 4312, Normandie Univ, UNIROUEN</institution>
, Evreux,<country>France</country>
</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Lesouhaitier, Olivier" sort="Lesouhaitier, Olivier" uniqKey="Lesouhaitier O" first="Olivier" last="Lesouhaitier">Olivier Lesouhaitier</name>
<affiliation wicri:level="1"><nlm:aff id="a1"><institution>Laboratory of Microbiology Signals and Microenvironment LMSM EA 4312, Normandie Univ, UNIROUEN</institution>
, Evreux,<country>France</country>
</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
</analytic>
<series><title level="j">Scientific Reports</title>
<idno type="eISSN">2045-2322</idno>
<imprint><date when="2017">2017</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>We have previously shown that the eukaryotic C-type natriuretic peptide hormone (CNP) regulates <italic>Pseudomonas aeruginosa</italic>
 virulence and biofilm formation after binding on the AmiC sensor, triggering the <italic>amiE</italic>
 transcription. Herein, the involvement of the aliphatic amidase AmiE in <italic>P. aeruginosa</italic>
 virulence regulation has been investigated. The proteome analysis of an AmiE over-producing strain (AmiE<sup>+</sup>
) revealed an expression change for 138 proteins, including some that are involved in motility, synthesis of quorum sensing compounds and virulence regulation. We observed that the AmiE<sup>+</sup>
 strain produced less biofilm compared to the wild type, and over-produced rhamnolipids. In the same line, AmiE is involved in <italic>P. aeruginosa</italic>
 motilities (swarming and twitching) and production of the quorum sensing molecules <italic>N</italic>
-acyl homoserine lactones and Pseudomonas Quinolone Signal (PQS). We observed that AmiE overproduction reduced levels of HCN and pyocyanin causing a decreased virulence in different hosts (i.e. <italic>Dictyostelium discoideum</italic>
 and <italic>Caenorhabditis elegans</italic>
). This phenotype was further confirmed in a mouse model of acute lung infection, in which AmiE overproduction resulted in an almost fully virulence decrease. Taken together, our data suggest that, in addition to its role in bacterial secondary metabolism, AmiE is involved in <italic>P. aeruginosa</italic>
 virulence regulation by modulating pilus synthesis and cell-to-cell communication.</p>
</div>
</front>
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</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Stiernagle, T" uniqKey="Stiernagle T">T. Stiernagle</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Blier, A S" uniqKey="Blier A">A. S. Blier</name>
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</TEI>
<affiliations><list><country><li>Allemagne</li>
<li>Australie</li>
<li>France</li>
<li>Irlande (pays)</li>
</country>
</list>
<tree><country name="France"><noRegion><name sortKey="Clamens, Thomas" sort="Clamens, Thomas" uniqKey="Clamens T" first="Thomas" last="Clamens">Thomas Clamens</name>
</noRegion>
<name sortKey="Bortolotti, Perrine" sort="Bortolotti, Perrine" uniqKey="Bortolotti P" first="Perrine" last="Bortolotti">Perrine Bortolotti</name>
<name sortKey="Bouffartigues, Emeline" sort="Bouffartigues, Emeline" uniqKey="Bouffartigues E" first="Emeline" last="Bouffartigues">Emeline Bouffartigues</name>
<name sortKey="Chevalier, Sylvie" sort="Chevalier, Sylvie" uniqKey="Chevalier S" first="Sylvie" last="Chevalier">Sylvie Chevalier</name>
<name sortKey="Cornelis, Pierre" sort="Cornelis, Pierre" uniqKey="Cornelis P" first="Pierre" last="Cornelis">Pierre Cornelis</name>
<name sortKey="Cosette, Pascal" sort="Cosette, Pascal" uniqKey="Cosette P" first="Pascal" last="Cosette">Pascal Cosette</name>
<name sortKey="Crepin, Alexandre" sort="Crepin, Alexandre" uniqKey="Crepin A" first="Alexandre" last="Crépin">Alexandre Crépin</name>
<name sortKey="Dufour, Alain" sort="Dufour, Alain" uniqKey="Dufour A" first="Alain" last="Dufour">Alain Dufour</name>
<name sortKey="Feuilloley, Marc G J" sort="Feuilloley, Marc G J" uniqKey="Feuilloley M" first="Marc G. J." last="Feuilloley">Marc G. J. Feuilloley</name>
<name sortKey="Grandjean, Teddy" sort="Grandjean, Teddy" uniqKey="Grandjean T" first="Teddy" last="Grandjean">Teddy Grandjean</name>
<name sortKey="Guery, Benoit" sort="Guery, Benoit" uniqKey="Guery B" first="Benoit" last="Guery">Benoit Guery</name>
<name sortKey="Hardouin, Julie" sort="Hardouin, Julie" uniqKey="Hardouin J" first="Julie" last="Hardouin">Julie Hardouin</name>
<name sortKey="Hillion, Melanie" sort="Hillion, Melanie" uniqKey="Hillion M" first="Mélanie" last="Hillion">Mélanie Hillion</name>
<name sortKey="Kentache, Takfarinas" sort="Kentache, Takfarinas" uniqKey="Kentache T" first="Takfarinas" last="Kentache">Takfarinas Kentache</name>
<name sortKey="Lesouhaitier, Olivier" sort="Lesouhaitier, Olivier" uniqKey="Lesouhaitier O" first="Olivier" last="Lesouhaitier">Olivier Lesouhaitier</name>
<name sortKey="Rosay, Thibaut" sort="Rosay, Thibaut" uniqKey="Rosay T" first="Thibaut" last="Rosay">Thibaut Rosay</name>
<name sortKey="Vieillard, Julien" sort="Vieillard, Julien" uniqKey="Vieillard J" first="Julien" last="Vieillard">Julien Vieillard</name>
</country>
<country name="Allemagne"><noRegion><name sortKey="Neidig, Anke" sort="Neidig, Anke" uniqKey="Neidig A" first="Anke" last="Neidig">Anke Neidig</name>
</noRegion>
<name sortKey="Overhage, Joerg" sort="Overhage, Joerg" uniqKey="Overhage J" first="Joerg" last="Overhage">Joerg Overhage</name>
</country>
<country name="Irlande (pays)"><noRegion><name sortKey="Mooij, Marlies" sort="Mooij, Marlies" uniqKey="Mooij M" first="Marlies" last="Mooij">Marlies Mooij</name>
</noRegion>
<name sortKey="O Ara, Fergal" sort="O Ara, Fergal" uniqKey="O Ara F" first="Fergal" last="O Ara">Fergal O Ara</name>
</country>
<country name="Australie"><noRegion><name sortKey="O Ara, Fergal" sort="O Ara, Fergal" uniqKey="O Ara F" first="Fergal" last="O Ara">Fergal O Ara</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>

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{{Explor lien
   |wiki=    Wicri/Asie
   |area=    AustralieFrV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     PMC:5259723
   |texte=   The aliphatic amidase AmiE is involved in regulation of Pseudomonas aeruginosa virulence
}}

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Serveur d'exploration sur les relations entre la France et l'Australie

The aliphatic amidase AmiE is involved in regulation of Pseudomonas aeruginosa virulence

The aliphatic amidase AmiE is involved in regulation of Pseudomonas aeruginosa virulence

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Abstract

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	Serveur d'exploration sur les relations entre la France et l'Australie
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